Anti-HDAC3 Antibody (Clone#EFH-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4434

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-HDAC3 antibody (BM4434). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HDAC3 antigen affinity purified monoclonal antibody (BM4434) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.
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产品简介>

产品名称

Anti-HDAC3 Antibody (Clone#EFH-8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HD3; HDAC3; histone deacetylase 3; RPD3; RPD3 2; SMAP45

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

HDAC3

克隆号

EFH-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human HDAC3

计算分子量

49 kDa

实际分子量

49 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HDAC3 (HISTONE DEACETYLASE 3) is a member of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC3 gene. The HDAC3 gene is mapped to 5q31.3. HDAC3 has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53% amino acid identity with HDAC1 and 52% with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4. HDAC3 recruitment to the genome displays a circadian rhythm in mouse liver.

Uniprot ID

O15379

文献引用格式

HDAC3 Antibody (Clone#EFH-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4434)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Histone deacetylase 3

实验方案>

实验步骤

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