Anti-HDAC3 Antibody

筛选器： All IHC ICC/IF WB FCM

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  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in frozen section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  ICC analysis of HDAC3 using anti- HDAC3 antibody (BA0916).
  HDAC3 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  Western blot analysis of HDAC3 using anti-HDAC3 antibody (BA0916). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat Stomach tissue lysates,
  Lane 2: Rat Testis tissue lysates,
  Lane 3: MCF-7 whole cell lysates,
  Lane 4: HELA whole cell lysates,
  Lane 5: JURKAT whole cell lysates,
  Lane 6: SKOV whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (BA0916) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.

• 

  IF analysis of HDAC3 using anti- HDAC3 antibody (BA0916).
  HDAC3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- HDAC3 Antibody (BA0916) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  IF analysis of HDAC3 using anti- HDAC3 antibody (BA0916).
  HDAC3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- HDAC3 Antibody (BA0916) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  Flow Cytometry analysis of A431 cells using anti-HDAC3 antibody (BA0916).
  Overlay histogram showing A431 cells stained with BA0916 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (BA0916) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of U937 cells using anti-HDAC3 antibody (BA0916).
  Overlay histogram showing U937 cells stained with BA0916 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (BA0916) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  IHC analysis of HDAC3 using anti-HDAC3 antibody (BA0916).
  HDAC3 was detected in frozen section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  ICC analysis of HDAC3 using anti- HDAC3 antibody (BA0916).
  HDAC3 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-HDAC3 Antibody (BA0916) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  Western blot analysis of HDAC3 using anti-HDAC3 antibody (BA0916). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat Stomach tissue lysates,
  Lane 2: Rat Testis tissue lysates,
  Lane 3: MCF-7 whole cell lysates,
  Lane 4: HELA whole cell lysates,
  Lane 5: JURKAT whole cell lysates,
  Lane 6: SKOV whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (BA0916) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.

• 

  IF analysis of HDAC3 using anti- HDAC3 antibody (BA0916).
  HDAC3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- HDAC3 Antibody (BA0916) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  IF analysis of HDAC3 using anti- HDAC3 antibody (BA0916).
  HDAC3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- HDAC3 Antibody (BA0916) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Flow Cytometry analysis of A431 cells using anti-HDAC3 antibody (BA0916).
  Overlay histogram showing A431 cells stained with BA0916 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (BA0916) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of U937 cells using anti-HDAC3 antibody (BA0916).
  Overlay histogram showing U937 cells stained with BA0916 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (BA0916) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.