Anti-CD34 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00885-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- Cd34 antibody (A00885-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: rat skeletal muscle tissue lysates,Lane 3: rat lung tissue lysates,Lane 4: mouse lung tissue lysates.Use rabbit anti- Cd34 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Cd34 at approximately 105KD. The expected band size for Cd34 at 105KD.
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Figure 2. IHC analysis using anti- Cd34 antibody (A00885-1). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis using anti- Cd34 antibody (A00885-1). detected in paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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产品简介>

产品名称

Anti-CD34 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

CD34; CD34 molecule

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB,IHC,ELISA

基因名称

CD34

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived rat CD34 recombinant protein (Position: S170-L386).

计算分子量

105 kDa

实际分子量

105-120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Uniprot ID

B1PLB1

RRID

AB_3081274

文献引用格式

CD34 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00885-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD34 molecule

蛋白名全称

Submitted name: CD34 antigen (Predicted)

实验方案>

实验步骤

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