Anti-CD34 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3414

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- CD34 antibody (BA3414). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
Use rabbit anti- CD34 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD34 at approximately 105-120KD. The expected band size for CD34 is at 41KD.
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Figure 2. IHC analysis using anti- CD34 antibody (BA3414). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis using anti- CD34 antibody (BA3414). detected in paraffin-embedded section of human tonsil tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CD34 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

CD34; CD34 molecule

产品类型

Polyclonal

检验物种

human, mouse ,rat

应用范围

WB,IHC

基因名称

CD34

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human CD34.

计算分子量

41 kDa

实际分子量

105-120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Uniprot ID

P28906

文献引用格式

CD34 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3414)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD34 molecule

蛋白名全称

Hematopoietic progenitor cell antigen CD34

实验方案>

实验步骤

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