Anti-CD34 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC IF ELISA

PB9053

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of anti- CD34 antibody (PB9053). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. Use rabbit anti- CD34 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
all(11)
Figure 2. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human lymphoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 3. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 4. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human glioma cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 5. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 6. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 7. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 8. IHC analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(11)
Figure 9. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(11)
Figure 10. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(11)
all(11)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD34 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

CD34; CD34 molecule

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, IHC, IF, ELISA

基因名称

CD34

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34.

计算分子量

41 kDa

实际分子量

105-120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Uniprot ID

P28906

RRID

AB_3081945

文献引用格式

CD34 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9053)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD34 molecule

蛋白名全称

Hematopoietic progenitor cell antigen CD34

实验方案>

实验步骤

引用文献(0)>

清除