Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti-GRP78/BIP/HSPA5 antibody (A00955). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (A00955) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 78 kDa.
Figure 1. Western blot analysis of anti-GRP78/BIP/HSPA5 antibody (A00955). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (A00955) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 78 kDa.