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Anti-GRP78/BIP/HSPA5 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

PB0669

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human PC-3 whole cell lysates,
    Lane 2: human MCF-7 whole cell lysates,
    Lane 3: human Hela whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: human U87 whole cell lysates,
    Lane 6: human Hacat whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (PB0669) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 72 kDa.

    all(6)
  • Figure 2. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
    GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(6)
  • Figure 3. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
    GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(6)
  • Figure 4. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
    GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(6)
  • Figure 5. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
    GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(6)
  • Figure 6. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
    GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(6)

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产品名称
Anti-GRP78/BIP/HSPA5 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
BIP; GRP 78; GRP78; GRP78/BIP; HSP A5; HSPA5; MIF2
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
HSPA5
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP identical to the related mouse and rat sequences.
计算分子量
72 kDa
实际分子量
78 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
HSPA5 (heat shock 70kDa protein 5), also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al. (2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER.
Uniprot ID
P11021  
文献引用格式
GRP78/BIP/HSPA5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0669)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
蛋白名全称
78 kDa glucose-regulated protein
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-GRP78/BIP/HSPA5 Antibody

    筛选器: All WB IHC

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