Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (PB0669) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 72 kDa.
Figure 2. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. Western blot analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (PB0669) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 72 kDa.
Figure 2. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (PB0669) .
GRP78/BIP/HSPA5 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-GRP78/BIP/HSPA5 Antibody (PB0669) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.