Anti-ZO-2/TJP2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

A02774-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- TJP2/ZO2 Antibody (A02774-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: COS-7 whole cell lysates,
Lane 4: PC-12 whole cell lysates.
Use rabbit anti- TJP2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TJP2 at approximately 150-170KD. The expected band size for TJP2 is at 134KD.
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Figure 2. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human Infiltrating adenocarcinoma of the lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human Papillary carcinoma of the left breast tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human This is a differentiated adenocarcinoma of the rectum tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 6. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 7. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 8. IHC analysis using anti- TJP2/ZO2 Antibody (A02774-2). detected in paraffin-embedded section of human Squamous metaplasia of the renal pelvis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 9. ICC analysis using anti- TJP2/ZO2 Antibody (A02774-2). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ZO-2/TJP2 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

Tight junction protein 2; Tight junction protein ZO 2; TJP2; X104; ZO 2; ZO2; ZO-2; Zona occludens protein 2; Zonula occludens protein 2

产品类型

Polyclonal

检验物种

human,rat,monkey

应用范围

WB,IHC,ICC/IF

基因名称

TJP2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TJP2/ZO2.

计算分子量

134 kDa

实际分子量

150 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

TJP2(Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2 is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins(TJPs) belong to a family of membrane-associated guanylate kinase(MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth(P0).

Uniprot ID

Q9UDY2

文献引用格式

ZO-2/TJP2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02774-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tight junction protein 2

蛋白名全称

Tight junction protein ZO-2

实验方案>

实验步骤

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