Anti-ZO-2/TJP2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BA2861

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of ZO-2/TJP2 using anti-ZO-2/TJP2 antibody (BA2861). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human U20S whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: human PC-3 whole cell lysates,
Lane 9: rat liver tissue lysates,
Lane 10: rat RH35 whole cell lysates,
Lane 11: mouse liver tissue lysates,
Lane 12: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ZO-2/TJP2 antigen affinity purified polyclonal antibody (BA2861) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ZO-2/TJP2 at approximately 150 kDa. The expected band size for ZO-2/TJP2 is at 134 kDa.
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Figure 2. IHC analysis of ZO-2/TJP2 using anti-ZO-2/TJP2 antibody (BA2861) .
ZO-2/TJP2 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-ZO-2/TJP2 Antibody (BA2861) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IF analysis of ZO-2/TJP2 using anti-ZO-2/TJP2 antibody (BA2861).
ZO-2/TJP2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ZO-2/TJP2 Antibody (BA2861) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Figure 4. IF analysis of ZO-2/TJP2 using anti-ZO-2/TJP2 antibody (BA2861).
ZO-2/TJP2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ZO-2/TJP2 Antibody (BA2861) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Figure 5. Flow Cytometry analysis of MCF-7 cells using anti-ZO-2/TJP2 antibody (BA2861).
Overlay histogram showing MCF-7 cells stained with BA2861 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZO-2/TJP2 Antibody (BA2861) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ZO-2/TJP2 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

Tight junction protein 2; Tight junction protein ZO 2; TJP2; X104; ZO 2; ZO2; ZO-2; Zona occludens protein 2; Zonula occludens protein 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

TJP2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TJP2.

计算分子量

134 kDa

实际分子量

150 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

TJP2(Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2 is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins(TJPs) belong to a family of membrane-associated guanylate kinase(MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth(P0).

Uniprot ID

Q9UDY2

文献引用格式

ZO-2/TJP2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2861)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tight junction protein 2

蛋白名全称

Tight junction protein ZO-2

实验方案>

实验步骤

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