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Anti-CD105/Endoglin/ENG Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A02997-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD105/Endoglin/ENG antigen affinity purified polyclonal antibody (A02997-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD105/Endoglin/ENG at approximately 95 kDa. The expected band size for CD105/Endoglin/ENG is at 71 kDa.

    all(8)
  • IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2).
    CD105/Endoglin/ENG was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2).
    CD105/Endoglin/ENG was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2).
    CD105/Endoglin/ENG was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2).
    CD105/Endoglin/ENG was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2).
    CD105/Endoglin/ENG was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-2).
    CD105/Endoglin/ENG was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-CD105/Endoglin/ENG Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD105; END; Endoglin; ENG; HHT1; ORW; ORW1
产品类型
Polyclonal
检验物种
mouse, rat
应用范围
WB, IHC, ELISA
基因名称
ENG
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived mouse CD105 recombinant protein (Position: E27-L226).
计算分子量
71 kDa
实际分子量
95 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Endoglin (Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.
Uniprot ID
Q63961  
RRID
文献引用格式
CD105/Endoglin/ENG Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02997-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
ELISA:1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

实验方案>

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    Anti-CD105/Endoglin/ENG Antibody

    筛选器: All WB IHC ELISA

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