Anti-CD105/Endoglin/ENG Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM ELISA

A02997-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- CD105/ENG antibody (A02997-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- CD105/ENG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CD105/ENG at approximately 95KD. The expected band size for CD105/ENG is at 71KD.
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IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-3).
CD105/Endoglin/ENG was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-3).
CD105/Endoglin/ENG was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-3).
CD105/Endoglin/ENG was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD105/Endoglin/ENG using anti-CD105/Endoglin/ENG antibody (A02997-3).
CD105/Endoglin/ENG was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- CD105/ENG antibody (A02997-3). detected in paraffin-embedded section of human ovarian cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).
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Flow Cytometry analysis of U87 cells using anti-CD105/Endoglin/ENG antibody (A02997-3).
Overlay histogram showing U87 cells stained with A02997-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD105/Endoglin/ENG Antibody (A02997-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD105/Endoglin/ENG Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD105; END; Endoglin; ENG; HHT1; ORW; ORW1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, IF, FCM, ELISA

基因名称

ENG

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD105/ENG recombinant protein (Position: E26-H267).

计算分子量

71 kDa

实际分子量

95 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Endoglin (Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.

Uniprot ID

P17813

文献引用格式

CD105/Endoglin/ENG Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02997-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

endoglin

蛋白名全称

Endoglin

实验方案>

实验步骤

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