Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- CDH1/E-cadherin antibody (BA0475). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
Use rabbit anti- CDH1/E-cadherin 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CDH1/E-cadherin at approximately 130KD. The expected band size for CDH1/E-cadherin is at 97KD.
Figure 1. Western blot analysis of anti- CDH1/E-cadherin antibody (BA0475). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
Use rabbit anti- CDH1/E-cadherin 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CDH1/E-cadherin at approximately 130KD. The expected band size for CDH1/E-cadherin is at 97KD.