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Anti-E-cadherin/CDH1 Antibody (Clone#OTI4F1)

Mouse monoclonal antibody

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筛选器: All IHC WB

M00063-5

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 2. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 4. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 5. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 6. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 10. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 11. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 12. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00063-5)

    all(13)
  • Figure 8. Western blot analysis of extracts (35ug) from 2 tissue lysates by using anti-CDH1 monoclonal antibody (1:500).

    all(13)
  • Figure 9. Western blot analysis of extracts (35ug) from 8 cell lines lysates by using anti-CDH1 monoclonal antibody (1:500).

    all(13)
  • Figure 13. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH1 (1:500).

    all(13)

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产品名称
Anti-E-cadherin/CDH1 Antibody (Clone#OTI4F1)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin
产品类型
Monoclonal
检验物种
human
应用范围
IHC, WB
基因名称
CDH1
克隆号
OTI4F1
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human CDH1 (NP_004351) produced in HEK293T cell.
计算分子量
97 kDa
实际分子量
130 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Uniprot ID
P12830  
文献引用格式
E-cadherin/CDH1 Antibody (Clone#OTI4F1) (Boster Biological Technology, Wuhan, China. Catalog#M00063-5)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
cadherin 1, type 1, E-cadherin(epithelial)
蛋白名全称
Cadherin-1
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150

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    Anti-E-cadherin/CDH1 Antibody (Clone#OTI4F1)

    筛选器: All IHC WB

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