Figure 1. IHC analysis using anti- Cytochrome C antibody (BA0781). detected in paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(8)
Figure 2. IHC analysis using anti- Cytochrome C antibody (BA0781). VCP was detected in frozen section of Rat Brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 3. IHC analysis using anti- Cytochrome C antibody (BA0781). VCP was detected in frozen section of A549 cell. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 4. Western blot analysis of anti- Cytochrome C antibody (BA0781).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver tissue lysates,Lane 2: Human hela whole cell lysates,Lane 3: Human MCF-7 whole cell lysates,Lane 4: mouse HEPA whole cell lysates.Use rabbit anti- Cytochrome C 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Cytochrome C at approximately 14KD. The expected band size for Cytochrome C is at 14KD.
all(8) 
Figure 5. IHC analysis of Cytochrome C using anti- Cytochrome C antibody (BA0781).
Cytochrome C was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytochrome C Antibody (BA0781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of Cytochrome C using anti- Cytochrome C antibody (BA0781).
Cytochrome C was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytochrome C Antibody (BA0781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. ICC analysis using anti- Cytochrome C antibody (BA0781). was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8) 
Figure 8. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(8) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. IHC analysis using anti- Cytochrome C antibody (BA0781). detected in paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis using anti- Cytochrome C antibody (BA0781). VCP was detected in frozen section of Rat Brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- Cytochrome C antibody (BA0781). VCP was detected in frozen section of A549 cell. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. Western blot analysis of anti- Cytochrome C antibody (BA0781).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver tissue lysates,Lane 2: Human hela whole cell lysates,Lane 3: Human MCF-7 whole cell lysates,Lane 4: mouse HEPA whole cell lysates.Use rabbit anti- Cytochrome C 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Cytochrome C at approximately 14KD. The expected band size for Cytochrome C is at 14KD.
Figure 5. IHC analysis of Cytochrome C using anti- Cytochrome C antibody (BA0781).
Cytochrome C was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytochrome C Antibody (BA0781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of Cytochrome C using anti- Cytochrome C antibody (BA0781).
Cytochrome C was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytochrome C Antibody (BA0781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. ICC analysis using anti- Cytochrome C antibody (BA0781). was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 8. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. IHC analysis using anti- Cytochrome C antibody (BA0781). detected in paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis using anti- Cytochrome C antibody (BA0781). VCP was detected in frozen section of Rat Brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- Cytochrome C antibody (BA0781). VCP was detected in frozen section of A549 cell. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. Western blot analysis of anti- Cytochrome C antibody (BA0781).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver tissue lysates,Lane 2: Human hela whole cell lysates,Lane 3: Human MCF-7 whole cell lysates,Lane 4: mouse HEPA whole cell lysates.Use rabbit anti- Cytochrome C 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Cytochrome C at approximately 14KD. The expected band size for Cytochrome C is at 14KD.
Figure 5. IHC analysis of Cytochrome C using anti- Cytochrome C antibody (BA0781).
Cytochrome C was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytochrome C Antibody (BA0781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of Cytochrome C using anti- Cytochrome C antibody (BA0781).
Cytochrome C was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytochrome C Antibody (BA0781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. ICC analysis using anti- Cytochrome C antibody (BA0781). was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 8. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
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