| Western blot (WB): | 1:500-2000 | 
| Immunohistochemistry (IHC): | 1:50-200 | 
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 | 
| ImmunoPrecipitation (IP): | 1:30 | 

Western blot analysis of anti-Cytochrome c/CYCS antibody (BM4571). The sample well of each lane was loaded with 30 ug of sample under reducing conditions. 
Lane 1: human Hela whole cell lysates, 
Lane 2: human 293T whole cell lysates, 
Lane 3: human HepG2 whole cell lysates, 
Lane 4: human HT-1080 whole cell lysates, 
Lane 5: rat kidney tissue lysates, 
Lane 6: rat skeletal muscle tissue lysates, 
Lane 7: mouse kidney tissue lysates, 
Lane 8: mouse skeletal muscle tissue lysates. 
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (BM4571) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.

Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.

Western blot analysis of anti-Cytochrome c/CYCS antibody (BM4571). The sample well of each lane was loaded with 30 ug of sample under reducing conditions. 
Lane 1: human Hela whole cell lysates, 
Lane 2: human 293T whole cell lysates, 
Lane 3: human HepG2 whole cell lysates, 
Lane 4: human HT-1080 whole cell lysates, 
Lane 5: rat kidney tissue lysates, 
Lane 6: rat skeletal muscle tissue lysates, 
Lane 7: mouse kidney tissue lysates, 
Lane 8: mouse skeletal muscle tissue lysates. 
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (BM4571) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.

Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.







