Anti-Cytochrome c/CYCS Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A03529

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of Cytochrome C using anti-Cytochrome C antibody (A03529). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat heart tissue lysate,Lane 3: mouse brain tissue lysate,Lane 4: mouse heart tissue lysate,Lane 5: human Hela whole Cell lysate,Lane 6: human MCF-7 whole Cell lysate,Lane 7: human 293T whole Cell lysate,Lane 8: human HepG2 whole Cell lysate,Lane 9: human Jurkat whole Cell lysate. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Cytochrome C at approximately 14KD. The expected band size for Cytochrome C is at 12KD.
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Figure 2. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529).Cytochrome C was detected in paraffin-embedded section of mouse brain tissue . . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529).Cytochrome C was detected in paraffin-embedded section of mouse intestine tissue . . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(5)
Figure 4. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529).Cytochrome C was detected in paraffin-embedded section of rat brain tissue . . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(5)
Figure 5. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529).Cytochrome C was detected in paraffin-embedded section of rat intestine tissue . . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(5)

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产品简介>

产品名称

Anti-Cytochrome c/CYCS Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

CYC; CYCS; Cytochrome c; cytochrome c; somatic; HCS; THC4

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB,IHC

基因名称

CYCS

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse Cytochrome C recombinant protein (Position: G2-E105). Mouse Cytochrome C shares 91.3% and 100% amino acid (aa) sequence identity with human and rat Cytochrome C, respectively.

计算分子量

12 kDa

实际分子量

14 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Uniprot ID

P62897

RRID

AB_3081637

文献引用格式

Cytochrome c/CYCS Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03529)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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