Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- AHSG antibody (BA4757-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta testis lysates,
Lane 2: HCCT whole cell lysates.
Use rabbit anti- AHSG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AHSG at approximately 58KD. The expected band size for AHSG is at 39KD.
Figure 1. Western blot analysis of anti- AHSG antibody (BA4757-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta testis lysates,
Lane 2: HCCT whole cell lysates.
Use rabbit anti- AHSG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AHSG at approximately 58KD. The expected band size for AHSG is at 39KD.