Anti-Fetuin A/AHSG Antibody

筛选器： All WB IHC FCM ELISA

• 

  Figure 1. Western blot analysis of anti- AHSG antibody (PB0598). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human placenta testis lysates,
  Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
  Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
  Lane 4: rat plasma testis lysates.
  Use rabbit anti- AHSG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AHSG at approximately 55-60KD. The expected band size for AHSG is at 39KD.

• 

  Figure 2. IHC analysis of AHSG using anti-AHSG antibody (PB0598).AHSG was detected in paraffin-embedded section of Human Liver Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AHSG Antibody (PB0598) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

• 

  Figure 3. Flow Cytometry analysis of HEPG2 cells using anti-Fetuin A antibody (PB0598).Overlay histogram showing HEPG2 cells stained with PB0598 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fetuin A Antibody (PB0598, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Figure 1. Western blot analysis of anti- AHSG antibody (PB0598). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human placenta testis lysates,
  Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
  Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
  Lane 4: rat plasma testis lysates.
  Use rabbit anti- AHSG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AHSG at approximately 55-60KD. The expected band size for AHSG is at 39KD.

• 

  Figure 2. IHC analysis of AHSG using anti-AHSG antibody (PB0598).AHSG was detected in paraffin-embedded section of Human Liver Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AHSG Antibody (PB0598) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

• 

  Figure 3. Flow Cytometry analysis of HEPG2 cells using anti-Fetuin A antibody (PB0598).Overlay histogram showing HEPG2 cells stained with PB0598 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fetuin A Antibody (PB0598, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

•