Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:150 |
Flow cytometry (FCM): | 1:100 |
Figure 1. Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-AHSG monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AHSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AHSG(Cat# M01251).
Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AHSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01251)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AHSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01251)
Figure 5. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AHSG antibody, and then analyzed by flow cytometry.
Figure 1. Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-AHSG monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AHSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AHSG(Cat# M01251).
Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AHSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01251)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AHSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01251)
Figure 5. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AHSG antibody, and then analyzed by flow cytometry.