Anti-Androgen receptor/AR Antibody (Clone#CDF-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4204

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Androgen receptor/AR antibody (BM4204). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Androgen receptor/AR antigen affinity purified monoclonal antibody (BM4204) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Androgen receptor/AR at approximately 110-120 kDa. The expected band size for Androgen receptor/AR is at 99 kDa.
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Immunohistochemical analysis of paraffin-embedded human prostate, using Androgen Receptor Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of MCF7 cells, using Androgen Receptor Antibody .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Androgen receptor/AR Antibody (Clone#CDF-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIS; androgen receptor; AR; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

克隆号

CDF-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Androgen Receptor

计算分子量

99 kDa

实际分子量

110-120 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The AR (androgen receptor) gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The AR gene is mapped to Xq12. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Uniprot ID

P10275

文献引用格式

Androgen receptor/AR Antibody (Clone#CDF-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4204)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Androgen receptor

实验方案>

实验步骤

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