产品名称
Anti-Androgen receptor/AR Antibody (Clone#DHH-1)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
AIS; androgen receptor; AR; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
免疫原
A synthesized peptide derived from human Androgen Receptor(AR-V7 specific)
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
The AR (androgen receptor) gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The AR gene is mapped to Xq12. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
文献引用格式
Androgen receptor/AR Antibody (Clone#DHH-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4349)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |