Anti-Androgen receptor/AR Antibody (Clone#OTI1D3)

Mouse monoclonal antibody

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筛选器： All IHC WB

MA00542

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00542) (1:150)
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Western blot analysis of extracts (10ug) from 1 cell lines by using anti-AR monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00542) (1:150)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AR (Cat# MA00542)(1:2000).
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Western blot analysis of extracts (35ug) from 2 different cell lines and 1human testis lyste by using anti-AR monoclonal antibody (PC3:human ; MCF7: human testis:human) (1:200).
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产品简介>

产品名称

Anti-Androgen receptor/AR Antibody (Clone#OTI1D3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIS; androgen receptor; AR; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

克隆号

OTI1D3

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 420-659 of human AR(NP_000035) produced in E.coli.

计算分子量

99 kDa

实际分子量

99 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot ID

P10275

文献引用格式

Androgen receptor/AR Antibody (Clone#OTI1D3) (Boster Biological Technology, Wuhan, China. Catalog#MA00542)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

androgen receptor

蛋白名全称

Androgen receptor

实验方案>

实验步骤

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