| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |

Western blot analysis of Anti-Phospho FOXO3 (S253 antibody (BM4401). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat brain tissue lysates,
Lane 3: Mouse brain tissue lysates,
Lane 4: Mouse brain tissue lysates,
Lane 5: Human T-47D whole cell lysates,
Lane 6: Human K562 whole cell lysates,
Lane 7: Human Caco-2 whole cell lysates,
Lane 8: Human PC-3 whole cell lysates.
Use rabbit Anti-Phospho FOXO3 (S253 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Phospho FOXO3 (S253 at approximately 97KD. The expected band size for Phospho FOXO3 (S253 is at 97KD.

Western blot analysis of Anti-Phospho FOXO3 (S253 antibody (BM4401). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat brain tissue lysates,
Lane 3: Mouse brain tissue lysates,
Lane 4: Mouse brain tissue lysates,
Lane 5: Human T-47D whole cell lysates,
Lane 6: Human K562 whole cell lysates,
Lane 7: Human Caco-2 whole cell lysates,
Lane 8: Human PC-3 whole cell lysates.
Use rabbit Anti-Phospho FOXO3 (S253 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Phospho FOXO3 (S253 at approximately 97KD. The expected band size for Phospho FOXO3 (S253 is at 97KD.


