Anti-FOXO3A/FOXO3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

PB9196

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (PB9196). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat ovary tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOXO3A/FOXO3 antigen affinity purified polyclonal antibody (PB9196) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOXO3A/FOXO3 at approximately 80-90 kDa. The expected band size for FOXO3A/FOXO3 is at 71 kDa.
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IHC analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (PB9196) .
FOXO3A/FOXO3 was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-FOXO3A/FOXO3 Antibody (PB9196) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (PB9196) .
FOXO3A/FOXO3 was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-FOXO3A/FOXO3 Antibody (PB9196) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (PB9196) .
FOXO3A/FOXO3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-FOXO3A/FOXO3 Antibody (PB9196) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (PB9196) and anti-Beta Tubulin antibody (M01857-3).
FOXO3A/FOXO3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-FOXO3A/FOXO3 Antibody (PB9196) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
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Flow Cytometry analysis of MCF-7 cells using anti-FOXO3A/FOXO3 antibody (PB9196).
Overlay histogram showing MCF-7 cells stained with PB9196 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXO3A/FOXO3 Antibody (PB9196, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-FOXO3A/FOXO3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AF6q21; AF6q21 protein; FKHRL1; FKHRL1P2; forkhead box O3; Forkhead box protein O3; FOXO2; FOXO3; FOXO3A

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

FOXO3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673). Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A.

计算分子量

71 kDa

实际分子量

80-100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Forkhead box O3, also known as FKHRL1 or FOXO3a, is a human protein encoded by the FOXO3 gene. FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA-binding domain. It is mapped to 6q21. This protein likely functions as a trigger for apoptosis through upregulation of genes necessary for cell death, such as Bim and PUMA, or downregulation of anti-apoptotic proteins such as FLIP. In mammals FOXO3 regulates the resistance of cells to stress by inducing DNA repair and thereby may also affect organismal life span. In addition, it is thought that FOXO3 is also involved in protection from oxidative stress by upregulating antioxidants such as catalase and MnSOD.

Uniprot ID

O43524

RRID

AB_3082014

文献引用格式

FOXO3A/FOXO3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9196)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

forkhead box O3

蛋白名全称

Forkhead box protein O3

实验方案>

实验步骤

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