Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXO3 (Cat# MA00252)(1:500).
all(4)
Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FOXO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00252) (1:150)
all(4) 
Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FOXO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00252) (1:150)
all(4) 
Figure 4. IF analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (MA00252).
FOXO3A/FOXO3 was detected in an immunocytochemical section of Hela cells. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.
| Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:150 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXO3 (Cat# MA00252)(1:500).
Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FOXO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00252) (1:150)
Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FOXO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00252) (1:150)
Figure 4. IF analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (MA00252).
FOXO3A/FOXO3 was detected in an immunocytochemical section of Hela cells. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.
Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXO3 (Cat# MA00252)(1:500).
Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FOXO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00252) (1:150)
Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FOXO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00252) (1:150)
Figure 4. IF analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (MA00252).
FOXO3A/FOXO3 was detected in an immunocytochemical section of Hela cells. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.
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