Anti-Caspase 8/CASP8 Antibody (Clone#EEG-3)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB

BM4423

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-CASP8 antibody (BM4423). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CASP8 antigen affinity purified monoclonal antibody (BM4423) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CASP8 at approximately 50 kDa. The expected band size for CASP8 is at 55 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Caspase 8/CASP8 Antibody (Clone#EEG-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptotic protease Mch 5; Casp 8; CASP8; CASP-8; Caspase 8; caspase8; FADD like ICE; FLICE; ICE like apoptotic protease 5; MCH5

产品类型

Monoclonal

检验物种

human

应用范围

基因名称

CASP8

克隆号

EEG-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Caspase-8

计算分子量

55 kDa

实际分子量

55 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Uniprot ID

Q14790

文献引用格式

Caspase 8/CASP8 Antibody (Clone#EEG-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4423)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Caspase-8

实验方案>

实验步骤

引用文献(0)>

清除