Anti-Caspase 8/CASP8 (p18) Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB

BA2143-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of Caspase 8/CASP8 (p18) using anti-Caspase 8/CASP8 (p18) antibody (BA2143-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat thymus tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: MCF-7 whole cell lysates,
Lane 4: HELA whole cell lysates,
Lane 5: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 8/CASP8 (p18) antigen affinity purified polyclonal antibody (BA2143-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 8/CASP8 (p18) at approximately 55 kDa. The expected band size for Caspase 8/CASP8 (p18) is at 55 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Caspase 8/CASP8 (p18) Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptotic protease Mch 5; Casp 8; CASP8; CASP-8; Caspase 8; caspase8; FADD like ICE; FLICE; ICE like apoptotic protease 5; MCH5

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

CASP8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-8(P18), different from the related mouse sequence by one amino acid.

计算分子量

55 kDa

实际分子量

55 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease(caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme(ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex(DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes.

Uniprot ID

Q14790

文献引用格式

Caspase 8/CASP8 (p18) Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2143-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

caspase 8, apoptosis-related cysteine peptidase

蛋白名全称

Caspase-8(CASP-8)

实验方案>

实验步骤

引用文献(0)>

清除