产品名称
Human Eotaxin/CCL11 ELISA Kit
指标别名
C C motif chemokine 11; CCL11; Eosinophil chemotactic protein; Eotaxin; SCYA11; Small inducible cytokine A11
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human Eotaxin/CCL11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0329)
背景资料
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0329-CAP | Anti-Human CCL11 Pre-coated 96-well strip microplate | 1 |
EK0329-ST | Human CCL11 Standard | 10ng/tube×2 |
EK0329-DA | Human CCL11 Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 83.4 | 205 | 468 | 84.5 | 202 | 485 |
Standard deviation | 3.5 | 12.5 | 19.2 | 8.2 | 19.8 | 44.1 |
CV(%) | 4.2 | 6.1 | 4.1 | 9.7 | 9.8 | 9.1 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.008 | 0.203 | 0.326 | 0.521 | 0.960 | 1.548 | 2.172 | 2.568 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (citrate, EDTA)