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Human Eotaxin/CCL11 ELISA Kit

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EK0329

  • 96T ¥2480
  • 货期: 现货

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产品简介>

产品名称
Human Eotaxin/CCL11 ELISA Kit
规格/价格
96T/2480
指标别名
C C motif chemokine 11; CCL11; Eosinophil chemotactic protein; Eotaxin; SCYA11; Small inducible cytokine A11
免疫原
E.coli, G24-P97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
CCL11
基因名全称
Eotaxin
Uniprot ID
P51671  
文献引用格式
Human Eotaxin/CCL11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0329)
背景资料
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0329-CAPAnti-Human CCL11 Pre-coated 96-well strip microplate1
EK0329-STHuman CCL11 Standard10ng/tube×2
EK0329-DAHuman CCL11 Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)83.420546884.5202485
Standard deviation3.512.519.28.219.844.1
CV(%)4.26.14.19.79.89.1
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0080.2030.3260.5210.9601.5482.1722.568
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
蛋白名全称
Eotaxin
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (citrate, EDTA)
实验时长
3.5h

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