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Mouse Eotaxin/CCL11 ELISA Kit

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EK0330

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse Eotaxin/CCL11 ELISA Kit
规格/价格
96T/2480
指标别名
C C motif chemokine 11; CCL11; Eosinophil chemotactic protein; Eotaxin; SCYA11; Small inducible cytokine A11
免疫原
Expression system for standard: E.coli; Immunogen sequence: H24-P97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6pg/ml-1000pg/ml
基因名称
CCL11
基因名全称
Eotaxin
Uniprot ID
P48298  
文献引用格式
Mouse Eotaxin/CCL11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0330)
背景资料
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
试剂盒内容
Catalog numberDescriptionQuantity
EK0330-CAP96-well plate precoated with anti- mouse Eotaxin antibody1
EK0330-STLyophilized recombinant mouse Eotaxin standard10ng/tube×2
EK0330-DABiotinylated anti- mouse Eotaxin antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3422340533223429
Standard deviation1.4911.5925.922.0712.7133.46
CV(%)4.4%5.2%6.4%6.3%5.7%7.8%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0640.1930.3310.5250.9901.3751.6781.952
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (citrate, EDTA)
实验时长
3.5h

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