产品名称
Rat Eotaxin/CCL11 ELISA Kit
指标别名
C C motif chemokine 11; CCL11; Eosinophil chemotactic protein; Eotaxin; SCYA11; Small inducible cytokine A11
免疫原
Expression system for standard: E.coli; Immunogen sequence: H24-P97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 11
文献引用格式
Rat Eotaxin/CCL11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1435)
背景资料
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
试剂盒内容
Catalog number | Description | Quantity |
EK1435-CAP | 96-well plate precoated with anti- rat Eotaxin antibody | 1 |
EK1435-ST | Lyophilized recombinant rat Eotaxin standard | 10ng/tube×2 |
EK1435-DA | Biotinylated anti- rat Eotaxin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 53 | 222 | 1050 | 58 | 464 | 1127Sta |
ndard | deviatio |
n | 3.71 | 12.88 | 64.05 | 3.07 | 19.49 | 66.50 |
CV(%) | 7% | 5.8% | 6.1% | 5.3% | 4.2% | 5.9% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.105 | 0.155 | 0.183 | 0.215 | 0.378 | 0.804 | 1.627 | 2.275 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA, citrate)