产品名称
Human MIP-1 Alpha/CCL3 ELISA Kit
指标别名
C C motif chemokine 3; CCL3; chemokine (C C motif) ligand 3; G0S19 1; LD78 alpha(4 69); LD78ALPHA; MIP 1 alpha; MIP1A; PAT 464.1; SCYA3; SIS beta; Small inducible cytokine A3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
chemokine (C-C motif) ligand 3
文献引用格式
Human MIP-1 Alpha/CCL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0448)
背景资料
Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0448-CAP | 96-well plate precoated with anti- human MIP-1 alpha antibody | 1 |
EK0448-ST | Lyophilized recombinant human MIP-1 alpha standard | 10ng/tubex2 |
EK0448-DA | Biotinylated anti- human MIP-1 alpha antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 175 | 370 | 30 | 166 | 361 |
Standard deviation | 2.54 | 10.32 | 14.8 | 2.58 | 12.45 | 20.93 |
CV(%) | 7.7 | 5.9 | 4 | 8.6 | 7.5 | 5.8 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.136 | 0.218 | 0.306 | 0.472 | 0.734 | 1.090 | 1.758 | 2.233 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)