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Human MIP-1 Alpha/CCL3 ELISA Kit

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EK0448

  • 96T ¥2280
  • 货期: 现货

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产品名称
Human MIP-1 Alpha/CCL3 ELISA Kit
规格/价格
96T/2280
指标别名
C C motif chemokine 3; CCL3; chemokine (C C motif) ligand 3; G0S19 1; LD78 alpha(4 69); LD78ALPHA; MIP 1 alpha; MIP1A; PAT 464.1; SCYA3; SIS beta; Small inducible cytokine A3
免疫原
E.coli, A27-A92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10 pg/ml
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
CCL3
基因名全称
chemokine (C-C motif) ligand 3
Uniprot ID
P10147  
文献引用格式
Human MIP-1 Alpha/CCL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0448)
背景资料
Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0448-CAP96-well plate precoated with anti- human MIP-1 alpha antibody1
EK0448-STLyophilized recombinant human MIP-1 alpha standard10ng/tubex2
EK0448-DABiotinylated anti- human MIP-1 alpha antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3317537030166361
Standard deviation2.5410.3214.82.5812.4520.93
CV(%)7.75.948.67.55.8
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.1360.2180.3060.4720.7341.0901.7582.233
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
实验时长
3.5h

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    Human MIP-1 Alpha/CCL3 ELISA Kit

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