产品名称
Rat MIP-1 Alpha/CCL3 ELISA Kit
指标别名
C C motif chemokine 3; CCL3; chemokine (C C motif) ligand 3; G0S19 1; LD78 alpha(4 69); LD78ALPHA; MIP 1 alpha; MIP1A; PAT 464.1; SCYA3; SIS beta; Small inducible cytokine A3
免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-A92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 3
文献引用格式
Rat MIP-1 Alpha/CCL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1219)
背景资料
Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
试剂盒内容
Catalog number | Description | Quantity |
EK1219-CAP | 96-well plate precoated with anti- rat MIP-1 alpha antibody | 1 |
EK1219-ST | Lyophilized recombinant rat MIP-1 alpha standard | 10ng/tube×2 |
EK1219-DA | Biotinylated anti- rat MIP-1 alpha antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 54 | 267 | 18 | 56 | 248 |
Standard deviation | 1.08 | 3.61 | 15.75 | 1.11 | 4.48 | 14.88 |
CV(%) | 6% | 6.7% | 5.9% | 6.2% | 8% | 6% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.137 | 0.218 | 0.283 | 0.422 | 0.691 | 1.101 | 1.816 | 2.423 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)