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Mouse MIP-1 Alpha/CCL3 ELISA Kit

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EK0449

  • 96T ¥2280
  • 货期: 现货

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产品名称
Mouse MIP-1 Alpha/CCL3 ELISA Kit
规格/价格
96T/2280
指标别名
C C motif chemokine 3; CCL3; chemokine (C C motif) ligand 3; G0S19 1; LD78 alpha(4 69); LD78ALPHA; MIP 1 alpha; MIP1A; PAT 464.1; SCYA3; SIS beta; Small inducible cytokine A3
免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-A92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10pg/ml
检测范围
7.8pg/ml-500pg/ml
基因名称
CCL3
基因名全称
C-C motif chemokine 3
Uniprot ID
P10855  
文献引用格式
Mouse MIP-1 Alpha/CCL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0449)
背景资料
Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
试剂盒内容
Catalog numberDescriptionQuantity
EK0449-CAP96-well plate precoated with anti- mouse CCL3 antibody1
EK0449-STLyophilized recombinant mouse CCL3 standard10ng/tubex2
EK0449-DABiotinylated anti- mouse CCL3 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)18742401869249
Standard deviation0.733.999.60.754.9614.44
CV(%)4.1%5.4%4%4.2%7.2%5.8%
典型数据示例
Concentration(pg/ml)07.815.631.362.5125250500
O.D.0.1060.1340.1660.2260.3450.5291.0281.704
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
实验时长
3.5h

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    Mouse MIP-1 Alpha/CCL3 ELISA Kit

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