产品名称
Mouse MIP-1 Alpha/CCL3 ELISA Kit
指标别名
C C motif chemokine 3; CCL3; chemokine (C C motif) ligand 3; G0S19 1; LD78 alpha(4 69); LD78ALPHA; MIP 1 alpha; MIP1A; PAT 464.1; SCYA3; SIS beta; Small inducible cytokine A3
免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-A92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 3
文献引用格式
Mouse MIP-1 Alpha/CCL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0449)
背景资料
Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0449-CAP | 96-well plate precoated with anti- mouse CCL3 antibody | 1 |
EK0449-ST | Lyophilized recombinant mouse CCL3 standard | 10ng/tubex2 |
EK0449-DA | Biotinylated anti- mouse CCL3 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 74 | 240 | 18 | 69 | 249 |
Standard deviation | 0.73 | 3.99 | 9.6 | 0.75 | 4.96 | 14.44 |
CV(%) | 4.1% | 5.4% | 4% | 4.2% | 7.2% | 5.8% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.106 | 0.134 | 0.166 | 0.226 | 0.345 | 0.529 | 1.028 | 1.704 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)