产品名称
Chicken Neurotrophin 3/NTF3 ELISA Kit
指标别名
HDNF; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; NGF 2; NGF2; NT 3; NT3; NTF3
免疫原
Expression system for standard: NS0; Immunogen sequence: Y139-T257
交叉反应
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
文献引用格式
Chicken Neurotrophin 3/NTF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0472-CH)
背景资料
Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1
试剂盒内容
Catalog number | Description | Quantity |
EK0472-CH-CAP | Anti-Chicken NTF3 Pre-coated 96-well strip microplate | 1 |
EK0472-CH-ST | Chicken NTF3 Standard | 10ng/tube×2 |
EK0472-CH-DA | Chicken NTF3 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 93 | 357 | 657 | 146 | 375 | 732 |
Standard deviation | 4 | 17.5 | 34.2 | 7.88 | 22.9 | 47.58 |
CV (%) | 4.3 | 4.9 | 5.2 | 5.4 | 6.1 | 6.5 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.014 | 0.051 | 0.073 | 0.148 | 0.277 | 0.561 | 0.970 | 1.886 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum