产品名称
Rat Neurotrophin 3/NTF3 ELISA Kit
指标别名
HDNF; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; NGF 2; NGF2; NT 3; NT3; NTF3
免疫原
Expression system for standard: NS0; Immunogen sequence: Y139-T257
交叉反应
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
文献引用格式
Rat Neurotrophin 3/NTF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0474)
背景资料
Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
试剂盒内容
Catalog number | Description | Quantity |
EK0474-CAP | 96-well plate precoated with anti- rat Neurotrophin-3 antibody | 1 |
EK0474-ST | Lyophilized recombinant rat Neurotrophin-3 standard | 10ng/tubex2 |
EK0474-DA | Biotinylated anti- rat Neurotrophin-3 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 41 | 173 | 464 | 41 | 177 | 504 |
Standard deviation | 2.25 | 12.45 | 23.66 | 2.99 | 15.93 | 27.72 |
CV(%) | 5.5% | 7.2% | 5.1% | 7.3% | 9% | 5.5% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.043 | 0.102 | 0.161 | 0.259 | 0.496 | 0.827 | 1.476 | 2.364 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum