产品名称
Mouse Neurotrophin 3/NTF3 ELISA Kit
指标别名
HDNF; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; NGF 2; NGF2; NT 3; NT3; NTF3
免疫原
Expression system for standard: NS0; Immunogen sequence: Y139-T257
交叉反应
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
文献引用格式
Mouse Neurotrophin 3/NTF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0473)
背景资料
Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0473-CAP | 96-well plate precoated with anti- mouse Neurotrophin-3 antibody | 1 |
EK0473-ST | Lyophilized recombinant mouse Neurotrophin-3 standard | 10ng/tubex2 |
EK0473-DA | Biotinylated anti- mouse Neurotrophin-3 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 31 | 195 | 532 | 31 | 208 | 563 |
Standard deviation | 2.29 | 7.8 | 29.79 | 2.85 | 9.36 | 33.78 |
CV(%) | 7.4% | 4% | 5.6% | 9.2% | 4.5% | 6% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.014 | 0.073 | 0.148 | 0.277 | 0.561 | 0.870 | 1.686 | 2.529 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum