Mouse Neurotrophin 3/NTF3 ELISA Kit

96T/2480

HDNF; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; NGF 2; NGF2; NT 3; NT3; NTF3

Expression system for standard: NS0; Immunogen sequence: Y139-T257

There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.

<2pg/ml

15.6pg/ml-1000pg/ml

NTF3

Neurotrophin-3

P20181

Mouse Neurotrophin 3/NTF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0473)

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

Catalog number	Description	Quantity
EK0473-CAP	96-well plate precoated with anti- mouse Neurotrophin-3 antibody	1
EK0473-ST	Lyophilized recombinant mouse Neurotrophin-3 standard	10ng/tubex2
EK0473-DA	Biotinylated anti- mouse Neurotrophin-3 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	31	195	532	31	208	563
Standard deviation	2.29	7.8	29.79	2.85	9.36	33.78
CV(%)	7.4%	4%	5.6%	9.2%	4.5%	6%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.014	0.073	0.148	0.277	0.561	0.870	1.686	2.529

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h