Human CD62L/SELL ELISA Kit

96T/1880

CD62L; gp90 MEL; hLHRc; LAM 1; LAM1; LECAM1; Leu 8; Leukocyte adhesion molecule 1; LNHR; LSEL; L-selectin; Lyam 1; LYAM1; Lymph node homing receptor; PLNHR; selectin L; SELL; TQ1

NSO, W39-N332

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/ml

62.5 pg/ml - 4000 pg/ml

SELL

selectin L

P14151

Human CD62L/SELL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0503)

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

20-25分钟

Catalog number	Description	Quantity
EK0503-CAP	96-well plate precoated with anti- human sL-Selectin antibody	1
EK0503-ST	Lyophilized recombinant human sL-Selectin standard	10ng/tubex2
EK0503-DA	Biotinylated anti- human sL-Selectin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	195	543	1239	187	595	1352
Standard deviation	15.21	33.66	60.71	16.64	37.48	67.6
CV(%)	7.8	6.2	4.9	8.9	6.3	5

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.008	0.116	0.225	0.407	0.753	1.292	1.780	2.340

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h