Mouse CD62L/SELL ELISA Kit

96T/1880

CD62L; gp90 MEL; hLHRc; LAM 1; LAM1; LECAM1; Leu 8; Leukocyte adhesion molecule 1; LNHR; LSEL; L-selectin; Lyam 1; LYAM1; Lymph node homing receptor; PLNHR; selectin L; SELL; TQ1

Expression system for standard: NS0; Immunogen sequence: W39-N332

There is no detectable cross-reactivity with other relevant proteins.

<5pg/ml

62.5pg/ml-4000pg/ml

SELL

selectin L

Q3TCF3

Mouse CD62L/SELL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0504)

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Catalog number	Description	Quantity
EK0504-CAP	96-well plate precoated with anti- mouse sL-Selectin antibody	1
EK0504-ST	Lyophilized recombinant mouse sL-Selectin standard	10ng/tubex2
EK0504-DA	Biotinylated anti- mouse sL-Selectin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	190	621	1771	204	669	1831
Standard deviation	9.5	32.29	138.13	11.01	34.78	157.46
CV(%)	5%	5.2%	7.8%	5.4%	5.2%	8.6%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.002	0.074	0.129	0.311	0.527	1.177	1.804	2.356

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h