Rat CD62L/SELL ELISA Kit

96T/2480

CD62L; gp90 MEL; hLHRc; LAM 1; LAM1; LECAM1; Leu 8; Leukocyte adhesion molecule 1; LNHR; LSEL; L-selectin; Lyam 1; LYAM1; Lymph node homing receptor; PLNHR; selectin L; SELL; TQ1

Expression system for standard: NS0; Immunogen sequence: W39-N332

There is no detectable cross-reactivity with other relevant proteins.

<300pg/ml

625pg/ml-40000pg/ml

SELL

selectin L

P30836

Rat CD62L/SELL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0937)

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

20-25分钟

Catalog number	Description	Quantity
EK0937-CAP	96-well plate precoated with anti- rat L-Selectin antibody	1
EK0937-ST	Lyophilized recombinant rat L-Selectin standard	40ng/tube×2
EK0937-DA	Biotinylated anti- rat L-Selectin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1474	3614	24600	1588	3716	23760
Standard deviation	89.91	166.24	1279.2	100.04	178.36	1663.2
CV(%)	6.1%	4.6%	5.2%	6.3%	4.8%	7%

Concentration(pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.004	0.550	0.919	1.411	1.799	2.074	2.129	2.226

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)