Bovine TGF Beta 1/TGFB1 ELISA Kit

96T/1880

CED; DPD1; LAP; TGF beta 1; tgf beta1; TGFB; TGFB1; TGFbeta; TGF-beta 1; tgfbeta1; tgf-beta1; TGFβ; TGF-β; TGFβ1; TGF-β1

Expression system for standard: CHO; Immunogen sequence: A279-S390

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

<1pg/ml

15.6pg/ml-1000pg/ml

TGFB1

Transforming growth factor beta-1

P01137  

Bovine TGF Beta 1/TGFB1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0513-BV)

Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

25-30分钟

Catalog number	Description	Quantity
EK0513-BV-CAP	Anti-Bovine TGFB1 Pre-coated 96-well strip microplate	1
EK0513-BV-ST	Bovine TGFB1 Standard	10ng/tube×2
EK0513-BV-DA	Bovine TGFB1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	38	184	433	36	179	490
Standard deviation	2.09	8.46	30.74	2.45	10.56	42.14
CV (%)	5.5%	4.6%	7.1%	6.8%	5.9%	8.6%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.075	0.136	0.229	0.310	0.462	0.770	1.192	1.837

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

bovine

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (EDTA) and Urine