Human LAP(TGF-beta1) ELISA Kit

96T/2480

CED; DPD1; LAP; TGF beta 1; tgf beta1; TGFB; TGFB1; TGFbeta; TGF-beta 1; tgfbeta1; tgf-beta1; TGFβ; TGF-β; TGFβ1; TGF-β1

sf21, L30-R278

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

62.5 pg/ml - 4000 pg/ml

TGFB1

transforming growth factor, beta 1

P01137

Human LAP(TGF-beta1) ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1218)

TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.

15-20分钟

Catalog number	Description	Quantity
EK1218-CAP	96-well plate precoated with anti- human LAP(TGF-beta1) antibody	1
EK1218-ST	Lyophilized recombinant human LAP(TGF-beta1) standard	10ng/tube×2
EK1218-DA	Biotinylated anti- human LAP(TGF-beta1) antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	87	673	2000	90	698	2085
Standard deviation	3.91	43.74	126	4.5	57.93	145.95
CV(%)	4.5	6.5	6.3	5	8.3	7

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.045	0.119	0.143	0.308	0.506	0.931	1.627	2.158

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h