Mouse TGF Beta 1/TGFB1 ELISA Kit

96T/1880

CED; DPD1; LAP; TGF beta 1; tgf beta1; TGFB; TGFB1; TGFbeta; TGF-beta 1; tgfbeta1; tgf-beta1; TGFβ; TGF-β; TGFβ1; TGF-β1

Expression system for standard: CHO; Immunogen sequence: A279-S390

There is no detectable cross-reactivity with other relevant proteins.

<2pg/ml

15.6pg/ml-1000pg/ml

TGFB1

Transforming growth factor beta-1

P04202

Mouse TGF Beta 1/TGFB1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0515)

Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Catalog number	Description	Quantity
EK0515-CAP	96-well plate precoated with anti- mouse TGF beta 1 antibody	1
EK0515-ST	Lyophilized recombinant mouse TGF beta 1 standard	10ng/tubex2
EK0515-DA	Biotinylated anti- mouse TGF beta 1 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	36	169	499	33	183	484
Standard deviation	1.47	11.66	31.43	1.55	12.99	34.36
CV(%)	4.1%	6.9%	6.3%	4.7%	7.1%	7.1%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.041	0.131	0.214	0.365	0.627	1.068	1.624	2.298

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (EDTA) and Urine

3.5h