Human AFP ELISA Kit

说明书

EK0651

96T ￥2480
货期：现货

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Human AFP ELISA Kit standard curve
all(2)
all(2)

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产品简介>

产品名称

Human AFP ELISA Kit

规格/价格

96T/2480

指标别名

AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP

免疫原

NSO, R19-V609

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10 pg/ml

检测范围

78 pg/ml - 5000 pg/ml

基因名称

AFP

基因名全称

alpha fetoprotein

Uniprot ID

P02771

文献引用格式

Human AFP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0651)

背景资料

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0651-CAP	96-well plate precoated with anti- human AFP antibody	1
EK0651-ST	Lyophilized recombinant human AFP standard	5ng/tube×2
EK0651-DA	Biotinylated anti- human AFP antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	95	643	2190	98	654	1986
Standard deviation	4.56	47.58	100.74	5.58	56.24	129.09
CV(%)	4.8	7.4	4.6	5.7	8.6	6.5

典型数据示例

Concentration(pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.003	0.137	0.248	0.486	0.777	1.260	1.704	1.925

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human AFP ELISA Kit

EK0651

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

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Human AFP ELISA Kit

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