Mouse AFP ELISA Kit

说明书

EK1661

96T ￥2480
货期：现货

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Mouse AFP ELISA Kit Standard Curve
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产品简介>

产品名称

Mouse AFP ELISA Kit

规格/价格

96T/2480

指标别名

AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP

免疫原

Expression system for standard: NS0; Immunogen sequence: K19-V605

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10pg/ml

检测范围

312pg/ml-20000pg/ml

基因名称

Afp

基因名全称

alpha fetoprotein

Uniprot ID

P02772

文献引用格式

Mouse AFP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1661)

背景资料

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

试剂盒内容

Catalog number	Description	Quantity
EK1661-CAP	96-well plate precoated with anti- mouse AFP antibody	1
EK1661-ST	Lyophilized recombinant mouse AFP standard	10ng/tube×2
EK1661-DA	Biotinylated anti- mouse AFP antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	50	189	834	57	204	928
Standard deviation	2.40	9.26	41.70	2.91	12.04	59.39
CV(%)	4.8%	4.9%	5.0%	5.1%	5.9%	6.4%

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.006	0.065	0.134	0.269	0.466	0.924	1.552	2.366

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse AFP ELISA Kit

EK1661

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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Mouse AFP ELISA Kit

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