| Description | Quantity | Volume | Buffers |
| Anti-Human AFP Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti-AFP monoclonal antibody, Polystyrene micro-well plate |
| Human AFP Standards (S0~S6) | 7 | 1ml | AFP (0, 5, 10, 20, 50, 100, 200) IU/mL, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, from natural protein |
| HRP Conjugated anti-Human AFP antibody | 1 | 7ml | HRP Conjugated anti-Human AFP antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300, from mouse monoclonal antibody |
| Controls | 2 | 1ml | L (10.5-19.5) H (46.3-86.0) IU/mL, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, from natural protein |
| 20X Wash Buffer Concentrate | 1 | 15ml | Phosphorous salts |
| Color Developing Reagent A | 1 | 7ml | Urea hydrogen peroxide |
| Color Developing Reagent B | 1 | 7ml | TMB |
| Stop Solution | 1 | 7ml | Dilute sulphuric acid |
| Plate Sealers | 2 | Piece |
| Concentration(IU/mL) | 0 | 5 | 10 | 20 | 50 | 100 | 200 |
| O.D. | 0.000 | 0.166 | 0.319 | 0.731 | 1.231 | 2.006 | 2.510 |
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