产品名称
Mouse MCP-3/CCL7 ELISA Kit
指标别名
C C motif chemokine 7; CCL7; chemokine (C C motif) ligand 7; FIC; MARC; MCP 3; MCP3; Monocyte chemotactic protein 3; NC28; SCYA6; SCYA7; Small inducible cytokine A7
免疫原
Expression system for standard: E.coli; Immunogen sequence: Q24-P97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 7
文献引用格式
Mouse MCP-3/CCL7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0683)
背景资料
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0683-CAP | 96-well plate precoated with anti- mouse CCL7 antibody | 1 |
EK0683-ST | Lyophilized recombinant mouse CCL7 standard | 10ng/tubex2 |
EK0683-DA | Biotinylated anti- mouse CCL7 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 194 | 459 | 31 | 176 | 492 |
Standard deviation | 1.51 | 13.19 | 30.75 | 1.58 | 14.08 | 42.31 |
CV(%) | 4.6% | 6.8% | 6.7% | 5.1% | 8% | 8.6% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.001 | 0.074 | 0.161 | 0.336 | 0.680 | 1.267 | 1.780 | 1.960 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)