产品名称
Mouse MCP-3/CCL7 Quick ELISA Kit
指标别名
C C motif chemokine 7; CCL7; chemokine (C C motif) ligand 7; FIC; MARC; MCP 3; MCP3; Monocyte chemotactic protein 3; NC28; SCYA6; SCYA7; Small inducible cytokine A7
免疫原
Expression system for standard: E.coli; Immunogen sequence: Q24-P97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 7
文献引用格式
Mouse MCP-3/CCL7 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0683)
背景资料
Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
试剂盒内容
Catalog number | Description | Quantity |
FEK0663-CAP | 96-well plate precoated with anti-Mouse CCL7 antibody | 1 |
FEK0663-ST | lyophilized recombinant Mouse CCL7 standard | 10ng/tube×2 |
FEK0663-A | Mouse CCL7 Antibody A | 3ml |
FEK0683-B | Mouse CCL7 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 196 | 461 | 33 | 178 | 494 |
Standard deviation | 1.50 | 12.74 | 28.12 | 2.90 | 13.35 | 43.47 |
CV(%) | 4.3% | 6.5% | 6.1% | 8.8% | 7.5% | 8.8% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
O.D. | 0.025 | 0.171 | 0.307 | 0.552 | 0.946 | 1.611 | 2.060 | 2.226 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)