产品名称
Human MCP-3/CCL7 Quick ELISA Kit
指标别名
C C motif chemokine 7; CCL7; chemokine (C C motif) ligand 7; FIC; MARC; MCP 3; MCP3; Monocyte chemotactic protein 3; NC28; SCYA6; SCYA7; Small inducible cytokine A7
免疫原
Expression system for standard: E.coli; Immunogen sequence: Q24-L99
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 7
文献引用格式
Human MCP-3/CCL7 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0443)
背景资料
Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
试剂盒内容
Catalog number | Description | Quantity |
FEK0443-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0443-ST | Human MCP-3 standard | 10ng/tube×2 |
FEK0443-A | Human MCP-3 Antibody A | 3ml |
FEK0443-B | Human MCP-3 Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 38 | 169 | 505 | 37 | 158 | 504 |
Standard deviation | 2.39 | 12.67 | 27.27 | 2.55 | 12.79 | 31.75 |
CV(%) | 6.3% | 7.5% | 5.4% | 6.9% | 8.1% | 6.3% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.019 | 0.081 | 0.135 | 0.236 | 0.416 | 0.750 | 1.425 | 2.307 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)