Catalog number | Description | Quantity |
---|---|---|
EK0980-CAP | 96-well plate precoated with anti- human Betacellulin antibody | 1 |
EK0980-ST | Lyophilized recombinant human Betacellulin standard | 10ng/tubex2 |
EK0980-DA | Biotinylated anti- human Betacellulin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 220 | 402 | 34 | 202 | 396 |
Standard deviation | 1.47 | 12.76 | 26.13 | 1.66 | 15.15 | 30.88 |
CV(%) | 4.1 | 5.8 | 6.5 | 4.9 | 7.5 | 7.8 |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.073 | 0.086 | 0.112 | 0.179 | 0.285 | 0.540 | 0.961 | 1.650 |
Human Betacellulin/BTC ELISA Kit standard curve
Human Betacellulin/BTC ELISA Kit standard curve