指标别名
betacellulin; BTC; Probetacellulin
免疫原
Expression system for standard: E.coli; Immunogen sequence: D32-Y111
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat BTC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1297)
背景资料
Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
试剂盒内容
Catalog number | Description | Quantity |
EK1297-CAP | 96-well plate precoated with anti- rat BTC antibody | 1 |
EK1297-ST | Lyophilized recombinant rat BTC standard | 10ng/tube×2 |
EK1297-DA | Biotinylated anti- rat BTC antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 15 | 51 | 280 | 16 | 55 | 280 |
Standard deviation | 0.6 | 3.72 | 14 | 0.92 | 4.29 | 17.64 |
CV(%) | 4% | 7.3% | 5% | 5.8% | 7.8% | 6.3% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.100 | 0.118 | 0.128 | 0.171 | 0.253 | 0.421 | 0.837 | 1.801 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)